Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: A549 and HFFF2 cells viability 24 h after incubation with nanomaterials, measured by three different assays. Statistically significant difference between a group and the control is marked as * p < 0.05, ** p < 0.0021, *** p < 0.0002, p **** < 0.0001. MTT and NR results are presented as % of control (mean with standard variation). Blue bars, GO; red bars, Ag; purple, GO:Ag. Notes: MTT is based on mitochondrial succinate dehydrogenase activity; NR is based on lysosomal uptake of neutral red. LDH is based on leakage of cytosol lactic dehydrogenase

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Incubation, Control, Activity Assay

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: A549 and HFFF2 cell proliferation rate after 24 h and 48 h of incubation with nanomaterials, measured by colorimetric BrdU assay. Statistically significant difference between a group and the control is marked as * p < 0.05, **** p < 0.0001. Results are presented as % of control (mean with standard variation). Blue bars, GO; red bars, Ag; purple, GO:Ag

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Incubation, BrdU Staining, Control

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: Reactive oxygen species (ROS) level in A549 and HFFF2 cells after 2 h of incubation with nanomaterials, measured by colorimetric DCF-DA fluorometric assay. Statistically significant difference between a group and the control is marked as ** p < 0.0021, *** p < 0.0002, p **** < 0.0001. Results are presented as mean fluorescence intensity (MFI) with standard deviation. Blue bars, GO; red bars, Ag; purple, GO:Ag

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Incubation, Control, Fluorescence, Standard Deviation

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: Caspase 3/7 activity in A549 and HFFF2 cells after 2 h of treatment with nanomaterials, measured by fluorometric CellEvent assay. There were no statistically significant differences. Results are presented as mean fluorescence intensity (MFI) with standard deviation. Blue bars, GO; red bars, Ag; purple, GO:Ag; green, additional controls

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Activity Assay, Fluorescence, Standard Deviation

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: Confocal imaging of HFFF2 cells combined with caspase 3/7 activity detection after treatment with nanomaterials for 2 h. Additional positive control: ketoconazole. Scale bar 450 µm. Notes: Nuclei were stained with DAPI (blue), caspase- 3/7 activity was visualized using a CellEvent probe (green) which stains nuclei in the presence of active caspases; actin F was stained with phalloidin conjugated with Alexa Fluor 633 (red)

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Imaging, Activity Assay, Positive Control, Staining

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: Selected magnified pictures from confocal imaging of A549 and HFFF2 cells combined with caspase- 3/7 activity detection after treatment with nanomaterials for 2 h. Additional positive controls: miconazole (A549) and ketoconazole (HFFF2). Scale bar 450 µm. Notes: Nuclei were stained with DAPI (blue); caspase 3/7 activity was visualized using a CellEvent probe (green, bright nuclei) which stains nuclei in the presence of active caspases; actin F was stained with phalloidin conjugated with Alexa Fluor 633 (red)

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Imaging, Activity Assay, Staining

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: Live imaging of A549 (upper row) and HFFF2 (lower row) cells after 7 days of treatment with nanomaterials, which were introduced into the culture medium after cell adhesion

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Imaging

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: Live imaging of HFFF2 cells after seeding on tissue culture plates coated with nanomaterials. Cells were grown for 24 h. Agglomerates of thicker GO flakes are visible. Scale bar 200 µm

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Imaging

Journal: Environmental Science and Pollution Research International

Article Title: The cytocompatibility of graphene oxide as a platform to enhance the effectiveness and safety of silver nanoparticles through in vitro studies

doi: 10.1007/s11356-023-30151-1

Figure Lengend Snippet: May–Grünwald and Giemsa staining of A549 (upper row) and HFFF2 (lower row) cells after 24 days after seeding on tissue culture plates coated with nanomaterials (selected images). Agglomerates of thicker GO flakes are visible in the A549 cell line; stained large and thin flakes are visible in the HFFF2 cell line. Scale bar 200 µm

Article Snippet: HFFF2, human fetal foreskin fibroblasts, from the European Collection of Authenticated Cell Cultures, catalogue no 86031405, is a cell line isolated from a 14 to 18 week old male fetus.

Techniques: Staining